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M8407 Sigma

Methotrexate hydrate

powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

Synonym: 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Companion Products and Reagents,
InChI Key   FPJYMUQSRFJSEW-ZOWNYOTGSA-N
product line   BioReagent
assay   ≥98% (HPLC)
form   powder
solubility   H2O: insoluble
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Recommended for use in cell culture and molecular biology applications at 0.01-300 μM in nucleoside-free cell culture medium to select for DHFR expression.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Biochem/physiol Actions

Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I make a solution of Methotrexate hydrate, Product M8407?
Methoxtrexate is soluble in 1M NaOH or NH4OH (10 mg/ml). For use in cell culture, sodium carbonate buffer (pH 9.0-9.5) can be used to dissolve methotrexate.
How do I store a stock solution of Methotrexate hydrate, Product M8407?
A sterile solution of methotrexate can be stored at 2-8 °C for approximately 30 days.
How do I sterilize a solution of Methotrexate hydrate, Product M8407?
A solution of methotrexate can be sterile filtered using a 0.22 mm filter
How often does Methotrexate hydrate, Product M8407, need to be added to the media during selection?
Fresh methotrexate should be added each time the culture media is changed.  Regardless of cell growth, most media will need to be changed at least once a week.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

Articles

ABC Transporters and Cancer Drug Resistance

Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a group of...
BioFiles 2007, 2.4, 7.
Keywords: Absorption, Cancer, Clinical, Cloning, Combinatorial synthesis, Diseases, Enzymology, Gene expression, Genetic, Immunology, Pharmaceutical, Reductions, Search, Transfection, Vectors

UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18
Keywords: Clinical, Pharmaceutical, Separation

Peer-Reviewed Papers
15

References

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Description

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06693 Timestrip Plus -20 °C
M9929 Methotrexate, meets USP testing specifications

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