EMAIL THIS PAGE TO A FRIEND
M8410 Sigma

Mineral oil

light oil (neat), BioReagent, suitable for mouse embryo cell culture

Purchase

Properties

Related Categories Cell Culture, Mouse Embryo Culture, Reagents and Media Supplements, Specialty Media Systems
product line   BioReagent
form   light oil (neat)
storage condition   protect from light
refractive index   n20/D 1.467(lit.)
density   0.84 g/mL at 25 °C(lit.)
suitability   suitable for mouse embryo cell culture

Description

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

Application

Recommended for overlaying mouse embryo cultures to prevent drying.

Caution

This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Packaging

1 L in glass bottle

100, 500 mL in glass bottle

5 mL in poly bottle

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
PY8047000
Flash Point(F) 
>233.6 °F
Flash Point(C) 
>112 °C
Protocols & Articles

Articles

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Peer-Reviewed Papers
15

References

Related Products

comparable product

Product #

Description

Add to Cart

M5310 Mineral oil, light oil ((neat)), BioXtra, suitable for mouse embryo cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?