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  • M8433 - Anti-phospho-c-Myc (pThr58/pSer62) antibody produced in rabbit

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M8433 Sigma

Anti-phospho-c-Myc (pThr58/pSer62) antibody produced in rabbit

affinity isolated antibody, liquid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human, mouse, rat
application(s)   immunohistochemistry: 10 - 20 μg/mL
  immunoprecipitation: 2 - 4 μg/mL
  western blot: 0.5-4 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   liquid
mol wt   antigen mol wt ~64-67 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MYC(4609)
mouse ... Myc(17869)
rat ... Myc(24577)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to residues around phospho-Thr58 and phospho-Ser62 of human c-Myc.

General description

The transcription factor c-Myc is a short-lived nuclear phosphoprotein involved in cell proliferation, differentiation, and cancer. Expression of c-Myc is strictly controlled by mitogens and is required for cell cycle entry. c-Myc binds to DNA and activates transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro at Ser62 by MAP Kinase, and in vivo at both Thr58 and Ser62 although the pathways and kinases acting in vivo have not been identified. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to stimulate transcription. c-Myc, a ~45 kDa protein that migrates with an apparent molecular weight of ~64-67kDa in reducing SDS-PAGE, appears to regulate multiple aspects of growth control; c-Myc promotes cell proliferation in the presence of Cdk inhibitors and induces apoptosis upon overexpression in serum deprived cells.

Physical form

In phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Specificity

Does not react with non-phosphorylated c-Myc.

Preparation Note

Purified by protein A and peptide affinity chromatography.

Price and Availability


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