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M8558 Sigma

Monoclonal Anti-MDM2 antibody produced in mouse

~2 mg/mL, clone MD-219, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to E1,E2,E3 Ligase,
species reactivity   mouse
does not react with   human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.5-2 μg/mL using whole extract of transfected 293T (human embryonal kidney) cells, expressing mouse MDM2
clone   MD-219, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 90 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Mdm2(17246)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant mouse MDM2.

General description

Transformed mouse 3T3 cell double minute 2 (MDM2) is an E3 ligase expressed in podocytes. It is overexpressed in lymphomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody shows predominantly nuclear and some cytoplasmic staining in cells.

Biochem/physiol Actions

Transformed mouse 3T3 cell double minute 2 (MDM2) acts as a negative regulator of the tumor suppressor p53. It also regulates the cell cycle and inflammatory activities.

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