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M8662 Sigma

Mineral oil

PCR Reagent

Purchase

Properties

Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
grade   PCR Reagent
form   liquid
packaging   pkg of (5VL = 1 bottle of 7.5 mL)
color   colorless
refractive index   n20/D 1.467(lit.)
density   0.84 g/mL at 25 °C(lit.)
foreign activity   DNase, RNase, protease, none detected
storage temp.   room temp

Description

Application

• For routine PCR amplifications.
• Minimizes evaporation of reactions run in PCR instruments without heated lids.

Features and Benefits

• Provided in a convenient 5X 1-vial pack size

Packaging

5 vials in poly bottle

Price and Availability


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
PY8047000
Flash Point(F) 
>233.6 °F
Flash Point(C) 
>112 °C
Protocols & Articles

Protocols

Amplification of DNA Using Jumpstart REDTaq DNA Polymerase D8187 Protocol

Note: The use of DMSO or formamide with JumpStart REDTaq DNA Polymerase is not recommended due to interference with the enzyme-antibody complex. Other co-solvents, solutes (e.g., salts) and extremes ...
Keywords: Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Amplification of DNA Using Jumpstart Taq DNA Polymerase D9307 Protocol

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...

Long and Accurate PCR Amplification of DNA D8045 Protocol

Effective denaturation is accomplished by the use of higher temperatures for shorter periods of time or by the use of co-solvents, such as dimethyl sulfoxide. Addition of DMSO in the reaction at a fi...

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...

Low Contaminant Amplification of DNA Using MTP Taq DNA Polymerase D7442 Protocol

Every precaution should be taken to avoid contamination of reagents with unknown/unwanted DNA. This includes the following:

Reduce Non-specific PCR Amplification- Jumpstart Taq Polymerase D4148 Protocol

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
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References

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