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M8787 Sigma

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

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Properties

Related Categories Buffers / Buffer Salts, Core Bioreagents, General Reagents, Life Science Reagents for PCR, Molecular Biology,
Quality Level   PREMIUM
grade   PCR Reagent
InChI Key   TWRXJAOTZQYOKJ-UHFFFAOYSA-L
form   liquid
packaging   vial of 1.5 mL
concentration   25 mM±1 mM
color   colorless
Featured Industry   Agriculture
Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, none detected
storage temp.   2-8°C

Description

Application

• For routine PCR amplifications.
• Optimization of MgCl2 concentration in PCR reactions for improved yield.

Suitability

Suitable for optimization of polymerase chain reactions.

Price and Availability


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Protocols & Articles

Articles

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Qualitative Multiplex PCR Assay for Assessing DNA Quality from FFPE Tissues and Other Sources of Damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is ...
Steve Michalik, Christopher Williams
LSI Edition 23
Keywords: AGE, Amplification, Cancer, Cell disruption, Clinical, Degradations, Forensic, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Whole genome amplification

Protocols

Assessing DNA Quality from FFPE Tissues and Other Sources of Damaged DNA with Qualitative Multiplex PCR Assay

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is ...
Keywords: AGE, Applications, Cell disruption, Degradations, Forensic, Methods, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequences

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Multiplex qPCR is a technique that simultaneously amplifies two or more target sequences in a single reaction. Analysis of multiple targets per sample allows for conservation of limited starting mate...
Keywords: AGE, Amplification, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Poisons, Polymerase chain reaction, Polymerase chain reaction - quantitative

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

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Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Agriculture | Plant Breeding Workflow

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

PCR Selection Guide

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Peer-Reviewed Papers
15

References

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