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M9130 Sigma

4-Methylumbelliferyl-β-D-glucuronide hydrate

≥98% (HPLC), BioReagent, for identification of transformed plants

Synonym: 4-Methylumbelliferyl-β-D-glucopyranosiduronic acid, MUG

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Description

General description

MUG is a chemical commonly used for the detection of transformed plants. MUG shows low levels of fluorescence until treatment with glucuronidase (GUS) encoded in transformed plants. Transformed plants can be identified by their MUG fluorescence.

Packaging

1 g in poly bottle

25, 100, 250 mg in poly bottle

Reconstitution

Product is soluble in molecular biology grade water at a concentration of 0.35 mg/ml.

Application

Suitable for fluorescent detection of glucuronidase gene expression in plants.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

4-Methylumbelliferyl β-D-glucuronide hydrate

β-Glucuronidase (GUS) from E. coli has become the reporter enzyme of choice for genetic plant research. 4-Methylumbelliferyl ®-D-glucuronide (MUG) is commonly used as a substrate for detecting GUS ge...
Keywords: Gene expression, Genetic, Melting

Fermentation: Enzymes, Substrates and SCFA

The influence of probiotics on the metabolic activity of intestinal microbiota may be evaulated by detecting bacterial enzymatic activity in fecal samples, specifically β-glucosidase, β-glucuronidase...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Absorption, Cancer, Carcinogens, Cell proliferation, Chromatography, Enzyme activity, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Metabolites, Prebiotics, Probiotics, Thin layer chromatography

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Apoptosis, Cancer, Capillary electrophoresis, Carcinogens, Cell culture, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, Genomics, High performance liquid chromatography, Immunology, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Microbiology, Molecular biology, Nucleic acid hybridization, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Polymerase chain reaction - quantitative, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

Peer-Reviewed Papers
15

References

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