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M9182 Sigma

Anti-Macrophage Colony Stimulating Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-M-CSF

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Hematopoietic Cytokines,
species reactivity   mouse
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   mouse ... Csf1(12977)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant mouse M-CSF expressed in Escherichia coli.

General description

Macrophage Colony Stimulating Factor (M-CSF) is a cytokine that plays a crucial role in host response to viral and fungal infections. It is secreted by fibroblasts, monocytes/macrophages and epithelial cells. M-CSF has been reported to the growth and differentiation of monocytes/macrophages. The M-CSF levels increase rapidly in response to fungal infections by Candida albicans and Aspergillus fumigatus. Reports suggest that M-CSF may act in an autocrine/paracrine manner to sustain HIV replication and increase the frequency by which the macrophages become infected with the virus. M-CSF may be a treatment option to combat infections associated with neutropenia. Anti-Macrophage Colony Stimulating Factor antibody specifically reacts with mouse M-CSF. The antibody shows approximately 5% cross-reactivity with recombinant human M-CSF.

Physical form

Lyophilized from a 0.2 μm-filtered solution of phosphate-buffered saline.

Specificity

By immunoblotting and ELISA, the antibody shows 5% cross-reactivity with recombinant human M-CSF.

Application

Anti-Macrophage Colony Stimulating Factor antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting the recommended concentration is 0.1-0.2 μg/ml. It is also suitable to neutralize the biological activity of mouse M-CSF in neutralization assays at a working concentration of 0.015-0.035 μg/ml. The antibody was used to neutralize M-CSF of murine embryonic fibroblasts.

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