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M9274 Sigma

Murashige and Skoog Basal Medium

plant cell culture tested, with sucrose and agar

Synonym: MS Basal Medium

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Formula variant

With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 42.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M9274) contains the micronutrients and vitamins of the original classic formulation plus sucrose and agar. It can be supplemented with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
2
Protocols & Articles

Protocols

Murashige Skoog - Plant Tissue Culture Protocol

M5524 – Murashige and Skoog basal salt mixture (MS) M0654 – Murashige and Skoog basal salt macronutrient solution M0529 – Murashige and Skoog basal salt micronutrient solution M2909 – Murashige and S...
Keywords: Mass spectrometry, Vitamins

Peer-Reviewed Papers
15

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