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M9274 Sigma

Murashige and Skoog Basal Medium

plant cell culture tested, with sucrose and agar

Synonym: MS Basal Medium

Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

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Formula variant

With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).

Quantity

Formulated to contain 42.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M9274) contains the micronutrients and vitamins of the original classic formulation plus sucrose and agar. It can be supplemented with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

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