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M9274 Sigma

Murashige and Skoog Basal Medium

plant cell culture tested, with sucrose and agar

Synonym: MS Basal Medium

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Formula variant

With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 42.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M9274) contains the micronutrients and vitamins of the original classic formulation plus sucrose and agar. It can be supplemented with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Protocols

Murashige Skoog - Plant Tissue Culture Protocol

M5524 – Murashige and Skoog basal salt mixture (MS) M0654 – Murashige and Skoog basal salt macronutrient solution M0529 – Murashige and Skoog basal salt micronutrient solution M2909 – Murashige and S...
Keywords: Mass spectrometry, Vitamins

Peer-Reviewed Papers
15

References

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