|Related Categories||Autophagy, Autophagy Inhibitors, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,|
|mp||~300 °C (dec.)(lit.)|
This product is soluble in DMF (10 mg/ml, may require gentle heating), yielding a clear, colorless solution. It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/ml with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA are best prepared fresh.
3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.
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Ready Made Solution, 2.5 mg/mL in DMSO (2.74 mM), from Streptomyces hygroscopicus
≥95% (HPLC), powder
from Penicillium funiculosum, ≥98% (HPLC and TLC)
Certificate of Analysis
Certificate of Origin
Autophagy is a highly regulated process by which long-lived proteins, organelles, and protein aggregates are captured within autophagosomes, which are then fused with lysosomes for degradation.1 Auto...
Linda Stephenson, Ph.D., Product Specialist, Sigma® Life Science
Biofiles, Vol. 8, No. 9
Keywords: Apoptosis, Autophagy, Cancer, Degradations, Metabolites, transformation
MG-2477, a new tubulin inhibitor, induces autophagy through inhibition of the Akt/mTOR pathway and delayed apoptosis in A549 cells. Viola G, Bortolozzi R, Hamel E, Moro S, et al. Biochem. Pharmacol. 83(1), 16-26, (2011)
Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity. McFarland AJ, Anoopkumar-Dukie S, et al. Arch. Toxicol. 86(2), 275-84, (2011)
Mammalian target of rapamycin regulates isoliquiritigenin-induced autophagic and apoptotic cell death in adenoid cystic carcinoma cells. Chen G, Hu X, Zhang W, Xu N, et al. Apoptosis 17(1), 90-101, (2011)
FT-NMR 1 (3), 217:A / RegBook 1 (2), 2471:F / Structure Index 1, 390:D:8
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