3-Methyladenine autophagy inhibitor

Properties

Related Categories	Autophagy, Autophagy Inhibitors, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Core Bioreagents, Nucleoside Analogs, Nucleosides and Nucleotides, Nucleosides, Nucleotides, Oligonucleotides, Research Essentials More...
Quality Level	PREMIUM
mp	~300 °C (dec.)(lit.)

Description

Preparation Note

This product is soluble in DMF (10 mg/ml, may require gentle heating), yielding a clear, colorless solution. It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/ml with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA are best prepared fresh.

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

Price and Availability

Related Papers

MG-2477, a new tubulin inhibitor, induces autophagy through inhibition of the Akt/mTOR pathway and delayed apoptosis in A549 cells. Viola G, Bortolozzi R, Hamel E, Moro S, et al. Biochem. Pharmacol. 83(1), 16-26, (2011)

Documents

Certificate of Analysis

Certificate of Origin

FT-IR Raman

FT-NMR

M9281 - Product Information Sheet (52 KB)

Safety Information

WGK Germany	3
RTECS	AU6520000

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Autophagy in Cancer Promotes Therapeutic Resistance

Autophagy is a highly regulated process by which long-lived proteins, organelles, and protein aggregates are captured within autophagosomes, which are then fused with lysosomes for degradation.1 Auto...
Linda Stephenson, Ph.D., Product Specialist, Sigma^® Life Science
Biofiles, Vol. 8, No. 9
Keywords: Apoptosis, Autophagy, Cancer, Degradations, Metabolites, transformation

Papers

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MG-2477, a new tubulin inhibitor, induces autophagy through inhibition of the Akt/mTOR pathway and delayed apoptosis in A549 cells. Viola G, Bortolozzi R, Hamel E, Moro S, et al. Biochem. Pharmacol. 83(1), 16-26, (2011)

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Targeting autophagy enhances BO-1051-induced apoptosis in human malignant glioma cells. Chu PM, Chen LH, Chen MT, Ma HI, et al. Cancer Chemother. Pharmacol. 69(3), 621-33, (2011)

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Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity. McFarland AJ, Anoopkumar-Dukie S, et al. Arch. Toxicol. 86(2), 275-84, (2011)

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Mammalian target of rapamycin regulates isoliquiritigenin-induced autophagic and apoptotic cell death in adenoid cystic carcinoma cells. Chen G, Hu X, Zhang W, Xu N, et al. Apoptosis 17(1), 90-101, (2011)

Abberant α-synuclein confers toxicity to neurons in part through inhibition of chaperone-mediated autophagy. Maria Xilouri et al PLoS ONE 4, e5515, (2009)

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Beil. 26,IV,3564

FT-NMR 1 (3), 217:A / RegBook 1 (2), 2471:F / Structure Index 1, 390:D:8