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M9564 Sigma

Drug-Nucleic Acid Interactions, Methods in Enzymology Vol 340

  •  ISBN-10 0-12-182241-9

  •  ISBN-13 978-0-12-182241-5

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publication info   J.B. Chaires and M.J. Waring, ed., Academic Press, 2001, 800 pp., hard cover
mfr. no.   Elsevier

Description

General description

This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.

Table of Contents

Section I. Biophysical Approaches
Analysis and Interpretations of Ligand-DNA Binding Isotherms,
Surface Plasmon Resonance Biosensor Analysis of RNA-Small Molecule Interactions,
Simultaneous Measurement of Binding Constants and Unwinding Angles by Gel Electrophoresis,
Linear and Circular Dichroism of Drug-Nucleic Acid Complexes,
Rapid Screening of Structurally Selective Ligand Binding to Nucleic Acids,
Calorimetric Techniques in the Study of High-Order DNA-Drug Interactions,
Volume Changes Accompanying Interactions of Ligands with Nucleic Acids,
Calculating Sequence-Dependent Melting Stability of Duplex DNA Oligomers
and Multiplex Sequence Analysis by Graphs
Themal Denaturation as Tool to Study DNA-Ligand Interactions
Kinetics of Binding of Hoechst Dyes to DNA Studied by Stopped-Flow Fluorescence Techniques,
Scanning Force Microscopy of Nucleic Acid Complexes.
Nuclear Magnetic Resonance Studies of Drug-Nucleic Complexes in Solution
Molecular Modeling of Drug-DNA Complexes: An Update
Section II - Chemical and Molecular Biological Approaches
Peptide Nucleic Acid Targeting of Double -Stranded DNA
Drug Interaction with Triple-Helical Nucleic Acid
Measurement of Covalent Drug-DNA Interactions at the Nucleotide Level in Cells at Pharmacologically Relevant Doses,
Targeting DNA through Covalent Interactions of Reversible Binding Drugs,
Chemical Cross-Linking of Drugs to DNA
High-Resolution Footprinting Studies of Drug-DNA Complexes Using Chemical and Enzymatic Probes,
Drug-RNA Footprinting
Footprinting Methods for Analysis of Pyrrole-Imidazole Polyamidce/DNA Complexes
High-Resolution Transcription Assay for Probing Drug-DNA Interactions at Individual Drug Sites
Use of DNA Molecules Substituted with Unnatural Nucleotides to Probe Specific Drug Interactions
Drug Interactions with Nucleosomes and Chromatin
Locating Cobalt-Binding Sites on DNA Using Restriction Endonucleases
Exploiting Anthracycline Scaffold for Designing DNA-Targeting Agents
Design, Synthesis, and Characterization of Polyintercalating Ligands
Section III - Enzymology and Biological Approaches
Targeting Telomeres and Telomerase
Rapid, High-Throughput Engineering of Sequence-Specific Zinc Finger DNA-Binding Proteins
DNA Relaxation and Cleavage Assays to Study Topoisomerase I Inhibitors
In Vitro Human Immunodeficiency Virus Type I Integrase Assays
Use of Xenopus Egg Extracts to Study Effects of DNA-Binding Drugs on Chromatin Assembly, Nuclear Assembly, and DNA Replication

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