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M9807 Sigma

Microcharacterization of Proteins, 2nd Edition

  •  ISBN-10 3-527-30084-8

  •  ISBN-13 978-3-527-30084-6

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publication info   R. Kellner, et al., ed., Wiley-VCH, 1999, 348 pp., soft cover
mfr. no.   Wiley

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General description

This thorough introduction and laboratory guide details the most recent developments in protein separation and characterization, fragmentation and micro sequencing. It offers a stimulating combination of basic concepts and practical applications and presents the latest information in the rapidly emerging area of protein characterization.

Table of Contents

Partial table of contents:  
OVERVIEW.  
Microcharacterization of Proteins.  
SAMPLE PREPARATION.  
Microseparation Techniques I: 2D-Gel-Electrophoresis.  
Microseparation Techniques II: Electroblotting.  
Chemical and Enzymatic Fragmentation of Proteins.  
AMINO ACID ANALYSIS.  
Amino Acid Analysis.  
PROTEIN SEQUENCE ANALYSIS.  
The Edman Degradation.  
Analyzing Posttranslational Protein Modifications.  
BIOANALYTICAL MASS SPECTROMETRY.  
Overview, Methods and Applications.  
Electrospray Mass Spectrometry.  
DATABASE ANALYSIS.  
Protein Sequences and Sequence Databases.
Computational Sequence Analysis.  
Index.

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