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M9943 Sigma

10X MTP Taq Buffer

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

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Properties

form   liquid
color   colorless
shipped in   wet ice
storage temp.   −20°C

Description

Application

DNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Sigma product D7442). Each lot of MTP Taq buffer undergoes strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Legal Information

MTP is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Low Contaminant Amplification of DNA Using MTP Taq DNA Polymerase D7442 Protocol

Every precaution should be taken to avoid contamination of reagents with unknown/unwanted DNA. This includes the following:

PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme h...
Keywords: AGE, Amplification, Applications, Electrophoresis, Evaporation, Gel electrophoresis, Gene expression, Methods, PAGE, Polymerase chain reaction, Sequences

Peer-Reviewed Papers
15

References

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