|Related Categories||Biochemicals and Reagents, Blood and Urine Chemistry Assay Kits, Cancer Metabolism, Cancer Research, Cell Biology,|
sufficient for 100 colorimetric or fluor|
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Cholesterol is an important component of mammalian cell membranes where it functions in intracellular transport, cell signaling, and maintaining membrane fluidity. Within the blood, cholesterol circulates as both the free acid and as cholesterol esters. Controlling serum cholesterol has an important therapeutic role as elevated cholesterol levels are associated with the development of atherosclerosis and cardiovascular pathologies. Recent evidence suggests a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.
The Cholesterol Quantitation Kit can also be used to determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. In this kit, total cholesterol concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present. This kit is suitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples.
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