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MBK1 Sigma

Maleimide Activated BSA, KLH Conjugation Kit

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Description

Caution

Alkaline pH values (above 7.5) may hydrolyze the maleimide group or generate side reactions with amines. Haptens must contain cysteine or a sulfhydryl group in the reduced state in order to react efficiently with the maleimide group.

Features and Benefits

• Conjugation and purification in under 3 hours
• BSA and KLH are pre-activated and ready to use
• Mild reaction conditions: pH 6.5-7.5 in aqueous buffer
• Fast gel filtration separation of conjugate and free hapten
• Prepare immunogen and antigen for immunoassay with one kit
• Contains sufficient reagents for 3 conjugations each of BSA and KLH. Each reaction can conjugate at least 4 mg of hapten.
• Contains reagents for coupling efficiency assay
• Complete protocols and references included

Principle

Sigma offers a kit for conjugating haptens to bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH). BSA and KLH have been used as protein carriers for haptens in immunization and immunoassay protocols. Immunogen is prepared with one carrier protein, typically KLH, while the antigen for immunoassay is prepared with another carrier, typically BSA. This eliminates the need to remove antibodies developed against the immunogen carrier protein from the antiserum before assay.

Specificity

KLH and BSA have been activated with succinimidyl-3-N-maleimidopropionic acid to provide a maleimide group at the end of a three atom spacer. Under approximately neutral conditions (pH 6.5-7.5) the maleimide will form a thioether bond with a sulfhydryl group, i.e., a cysteine residue on a peptide.

Price and Availability


Detect Your Unseen Protein

Kit component also available separately

Description

Product #

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Maleimide Activated BSA 3 x 5 mg SDS B7542
Maleimide Activated KLH 3 x 5 mg SDS K0383
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

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Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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References

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