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MCL1 Sigma

Mammalian Cell Lysis Kit

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Properties

Related Categories Molecular Biology, Native Protein Sample Preparation, Proteomics, Recombinant Protein Extraction
usage   1 kit sufficient for 250 extractions (100 mm tissue culture plates)
shipped in   dry ice
storage temp.   −20°C

Description

Components

The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.

Features and Benefits

• The kit can be used to prepare cell lysis buffer and other modified buffers
• The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein

Application

The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.

Price and Availability


Ultrafree Centrifugal Filter Units

Kit component only

Description

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5X buffer (Tris-EDTA) 50 mL SDS    
5X DOC (Deoxycholic acid sodium salt) 50 mL SDS    
5X Igepal CA 630 50 mL SDS    
5X SDS (Sodium dodecyl sulfate) 50 mL SDS    
5X Sodium Chloride 50 mL SDS    

Standard component

Description

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Protease inhibitor cocktail 2.5 mL SDS P8340
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NA 1993 / PGIII
Protocols & Articles
Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?