|Related Categories||DNA Methylation Analysis, Epigenetics, Kits for Epigenetics, Molecular Biology|
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• ELISA based format
• Procedure completes in 4 easy to follow steps
• All reagents supplied including control methylated DNA
• Detection limit is 5 ng of fully methylated DNA
• Input DNA may be as low as 10-200 ng
• Procedure completes in 3.5 hours
• 96 well format allows option of studying single samples or high-throughput studies
• Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
• May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.
Imprint is a registered trademark of Sigma-Aldrich Co. LLC
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|10x Wash Buffer|
|DNA Binding Solution|
|8 well Assay Strips|
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The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 6
Keywords: Capture ELISA, Digestions, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Methylations, PAGE
Note: Storage Temperature - see Storage/Stability section for individual component storage conditions
Maternal dietary protein restriction and excess affects offspring gene expression and methylation of non-SMC subunits of condensin I in liver and skeletal muscle. Altmann S, et al. Epigenetics 7(3), 239-52, (2012)
Plasma S-adenosylhomocysteine is a better biomarker of atherosclerosis than homocysteine in apolipoprotein E-deficient mice fed high dietary methionine. Liu, C., et al. J. Nutr. 138(2), 311-315, (2008)
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