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MINI67 Sigma

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

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For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

Distributed for Phanos Technologies, Inc.

CellVue is a registered trademark of PTI Research, Inc.

Application

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

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Diluent C 10 mL SDS    
PKH67 Cell Linker in ethanol .1 mL SDS    
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
WGK Germany 
3
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C
Protocols & Articles

Articles

Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research

Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune c...
Katharine A. Muirhead§, Joseph D. Tario, Jr.†, Paul K. Wallace†
Biowire Spring 2011 — Cell Lines — Models of Disease
Keywords: Adhesion, Asymmetric synthesis, Cancer, Capillary electrophoresis, Cell disruption, Cell division, Cell proliferation, Clinical, Flow cytometry, Gene expression, Immunology, PAGE, Polymerase chain reaction, Solvents, Titrations

Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research

From our library of Articles, Sigma-Aldrich presents Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research
Keywords: Antivirals, Applications, Biowire, Buffers, Bulletins, Cancer, Capillary electrophoresis, Cell disruption, Cell proliferation, Clinical, Flow cytometry, Immunology, Methods, Molecular probes, Protocols, Type

PKH Bibliography

U.S. Patent 5,665,328 Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of PTI Research, Inc. used under license.
Keywords: AGE, Adhesion, Amplification, Angiogenesis, Antibiotics, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Biomaterials, Cancer, Capillary electrophoresis, Cell culture, Cell disruption, Cell division, Cell proliferation, Central Nervous System, Clinical, Eliminations, Endocrinology, Filtration, Flow cytometry, Gene expression, Growth factors, Immobilization, Immunology, Inflammation, Ion-exchange chromatography, Ligands, Magnetic resonance imaging, Mass spectrometry, Metabolites, Microscopy, Molecular biology, Reductions, Rheumatology, Transduction, Western blot

PKH Linker Kits

Many key research findings in cell function and pathogenesis have relied on labeling live cells. To study individual cell behavior, growth and differentiation, and cell-cell interactions, it is criti...
BioFiles 2007, 2.5, 22.
Keywords: Adhesion, Apoptosis, Cancer, Cell biology, Cell proliferation

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

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Keywords: Cell division, Immunofluorescence

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Cell Division Monitoring using Cell Tracking Dyes [VIDEO]

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Althou...
Keywords: Cancer, Cell proliferation, Clinical, Flow cytometry

Peer-Reviewed Papers
15

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