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MIRRT Sigma

MystiCq® microRNA cDNA Synthesis Mix

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Related Categories Molecular Biology, PCR/Amplification, Quantitative PCR, microRNA Quantitative PCR, microRNA cDNA Synthesis Mix More...
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Separate polyadenylation and reverse transcription steps allow for the possibility of optimization of protocol if necessary
• cDNA produced may be archived for future use
• Single RT reaction can provide up to 1000 qPCR reactions
• Includes a positive control primer for use with most human tissues

General description

Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR. Since microRNAs are not naturally polyadenylated, the first step in the cDNA synthesis process is to polyadenylate the microRNAs through a poly(A) polymerase reaction. Next, the ReadyScript reverse transcriptase, an oligo-dT adapter primer and other required reagents are added to convert the polyadenylated microRNAs into cDNA. The adapter primer includes a unique sequence at the 5’ end that is complementary to the sequence of the MystiCq Universal PCR Primer, another component of the MystiCq microRNA qPCR Assay System.The kit also contains a Positive Control Primer that is specific to SNORD44, a small nucleolar RNA that is widely expressed in most human tissues. Sufficient amounts of the Poly(A) Tailing Buffer and the MicroRNA cDNA Reaction mix are included in the kit to allow for the use of no poly(a) polymerase and no reverse transcriptase control reactions. To analyze individual microRNAs, use the MystiCq Universal PCR Primer (which targets the adapter primer sequence) together with the MystiCq microRNA qPCR Assay Primer and the MystiCq microRNA SYBR Green qPCR ReadyMix.

Packaging

Default reaction volume is 20 μL

Application

MystiCq® microRNA cDNA Synthesis Mix has been used for the quantitation of micro RNA-quantitative real time PCR (qRT-PCR).

Legal Information

MystiCq is a registered trademark used under license.

Price and Availability


All labs need water

Kit component only

Description

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Poly (A) Tailing Buffer (5X) SDS    
Human Positive Control Primer SDS    
Nuclease-free Water SDS    
MystiCq® Universal PCR Primer SDS    
Poly (A) Polymerase SDS    
microRNA cDNA Reaction Mix SDS    
ReadyScript® Reverse Transcriptase SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Quantitative PCR - PCR Technologies Guide

As described in Introduction and Historical Timelines and Polymerase Chain Reaction, PCR is currently considered to be the most useful technique that is applied to investigations using molecular biol...
Keywords: Amplification, Buffers, Clinical, Clinical Chemistry, Detection methods, Electrophoresis, Enzyme activity, Gel electrophoresis, Gene expression, Melting, Methylations, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Size-exclusion chromatography, Transcription

Protocols

Imprint RIP | uRNA Quantitation | MicroRNA primers | MicroRNA Reverse Transcription

Sigma’s Imprint RNA Immunoprecipitation Kit was used to co-purify human argonaute 2 (Ago2)-associated RNAs from HeLa cells, essentially as instructed in the Technical Bulletin. To evaluate the differ...
Carol Kreader
Principal R&D Scientist
Keywords: Buffers, Cell disruption, Immunoprecipitation, Polymerase chain reaction - quantitative, RNA immunoprecipitation, Western blot

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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