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MITOISO1 Sigma

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Features and Benefits

• Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
• Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
• Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
• Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Price and Availability


Flow Cytometry Instruments

Ultrafree Centrifugal Filter Units

Kit component only

Description

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Extraction buffer A 5X 50 mL SDS    
Bovine Serum Albumin solution 10 mL SDS    
Extraction buffer B, 5× 50 mL SDS    
JC-1 Stain 25 μg SDS    
JC-1 Assay buffer 5X 25 mL SDS    
Storage buffer 5X 25 mL SDS    
Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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