• USA Home
  • MITOISO1 - Mitochondria Isolation Kit

EMAIL THIS PAGE TO A FRIEND
MITOISO1 Sigma

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Features and Benefits

• Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
• Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
• Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
• Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Price and Availability


Ultrafree Centrifugal Filter Units

All labs need water

Biomedical Applications

Kit component only

Description

Product #

Add to Cart

Extraction buffer A 5X 50 mL SDS    
Bovine Serum Albumin solution 10 mL SDS    
Extraction buffer B, 5× 50 mL SDS    
JC-1 Stain 25 μg SDS    
JC-1 Assay buffer 5X 25 mL SDS    
Storage buffer 5X 25 mL SDS    
Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?
It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose.  This kit was originally designed for isolation from tissues.
Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?
The extraction buffers supplied with this kit are only available with the kit.
When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?
If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.
How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?
The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin.  We recommend a minimum of 50 mg.  To view this information on the MITOISO1 Technical Bulletin, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/mitoiso1bul.Par.0001.File.tmp/mitoiso1bul.pdf
Is the Product MITOISO1, Mitochondria Isolation Kit,  JC-1 stain available separately?
Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Fluka Product No. 40882.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?
Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit: If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.
Show more questions
Protocols & Articles

Articles

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Comments

Add to Cart

CYTOCOX1 Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria For the determination of cytochrome c oxidase activity in mitochondrial soluble and membrane bound samples.
CS0720 Citrate Synthase Assay Kit,  kit sufficient for 100 reactions (using a 1 ml cuvette),  kit sufficient for 480 reactions (using 96 multiwell plates)
MITOISO2 Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells For the isolation of enriched mitochondrial fraction from cells.
CS0760 Isolated Mitochondria Staining Kit,  kit sufficient for 50 reactions (in a 2 mL cuvette),  kit sufficient for 1,000 reactions (using 96 multiwell plates) Determine integrity of inner mitochondrial membrane.
MITOISO3 Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells For the isolation of enriched mitochondrial fraction from yeast.
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?