|Related Categories||Apoptosis Detection, Apoptosis and Cell Cycle, Biochemicals and Reagents, Cell Biology, Cell Fractionation and Organelle Isolation,|
|usage||sufficient for 50 applications (2-5 x 107 cells)|
|shipped in||dry ice|
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The Mitochondria Isolation Kit provides a fast and easy method for the isolation of an enriched mitochondrial fraction from cells. Most of the isolated mitochondria will contain inner and outer membranes. The kit contains extraction buffers for both functional studies and proteome profiling. Also included is a storage buffer for intact mitochondria.
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|Extraction buffer A 5X 25 mL|
|Storage buffer 5X 10 mL|
|CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL|
|Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL|
|Cell Lysis Solution .6 mL|
|Protein Extraction Reagent Type 4 1 bottle|
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Certificate of Analysis
Certificate of Origin
Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot
Graham, J.M., and Rickwood, D. Subcellular Fractionation, A Practical Approach New York, NY , (1997), 107-142
Activation of murine microglial cell lines by lipopolysaccaride and interferon-γ causes NO-mediated decreases in mitochondrial and cellular function. Moss, D.W., and Bates, T.E. Eur. J. Neurosci. 13, 529-538, (2001)
Facilitated mitochondrial import of antiviral and anticancer nucleoside drugs by human equilibrative nucleoside transporter-3 Govindarajan, R., et al., Am. J. Physiol. Gastrointest. Liver Physiol. 296(4), G910-G922, (2009)
Cell-derived soluble oligomers of human amyloid-β peptides disturb cellular homeostasis and induce apoptosis in primary hippocampal neurons. Yang, T.-T., et al. J. Neural Transm. Gen. Sect. 116(12), 1561-1569, (2009)
Amyloid precursor protein, heat-shock proteins, and Bcl-2 form a complex in mitochondria and modulate mitochondria function and apoptosis in N2a cells. Yang, T.-T., et al. Mech. Ageing Dev. 130(9), 592-601, (2009)
Hoechst 33342 induced reactive oxygen species and impaired expression of cytochrome c oxidase subunit 1 leading to cell death in irradiated human cancer cells. Athar, M., et al. Mol. Cell Biochem. 352(1-2), 281-292, (2011)
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|MITOISO1||Mitochondria Isolation Kit, sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues||For the isolation of enriched mitochondrial fraction from animal tissues.|
|CYTOCOX1||Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria||Determine integrity of outer mitochondrial membrane|
|CS0720||Citrate Synthase Assay Kit, kit sufficient for 100 reactions (using a 1 ml cuvette), kit sufficient for 480 reactions (using 96 multiwell plates)|
|CS0760||Isolated Mitochondria Staining Kit, kit sufficient for 50 reactions (in a 2 mL cuvette), kit sufficient for 1,000 reactions (using 96 multiwell plates)||Determine integrity of inner mitochondrial membrane,|
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