Yeast Mitochondria Isolation Kit
sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells
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Properties
| Related Categories | Apoptosis Detection, Apoptosis and Cell Cycle, Biochemicals and Reagents, Cell Biology, Cell Fractionation and Organelle Isolation, |
| usage | sufficient for 40 applications (using 20 OD culture preparations) |
| storage temp. | −20°C |
Description
Application
The kit contains all the reagents required for yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation. In addition, the kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies and a storage buffer for use with intact mitochondria.
Features and Benefits
• The kit′s features include speed, sensitivity, ease of use, and specificity
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe
General description
Mitochondria, the site of most of the energy production in eukaryotic cells, are characterized by a double membrane structure, an outer membrane and folded inner membrane. Isolated mitochondria may be used as tools for in vitro studies such as respiration and energy production studies, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling.
Price and Availability
Kit component also available separately
Kit component only
|
Description |
Product # |
Add to Cart |
|---|---|---|
| Storage buffer 5X | ||
| Buffer A 10x | ||
| Buffer B 2x | ||
| Cell Lysis Solution .6 mL |
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