|Related Categories||Apoptosis Detection, Apoptosis and Cell Cycle, Biochemicals and Reagents, Cell Biology, Cell Fractionation and Organelle Isolation,|
|usage||sufficient for 40 applications (using 20 OD culture preparations)|
The kit contains all the reagents required for yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation. In addition, the kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies and a storage buffer for use with intact mitochondria.
• The kit′s features include speed, sensitivity, ease of use, and specificity
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe
Mitochondria, the site of most of the energy production in eukaryotic cells, are characterized by a double membrane structure, an outer membrane and folded inner membrane. Isolated mitochondria may be used as tools for in vitro studies such as respiration and energy production studies, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling.
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|Storage buffer 5X|
|Buffer A 10x|
|Buffer B 2x|
|Cell Lysis Solution .6 mL|
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sufficient for 100 tests, soluble and membrane bound mitochondria
lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret
lyophilized powder, ≥200 units/mg solid
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
Certificate of Analysis
|Symbol||GHS06, GHS08, GHS09|
|Hazard statements||H301 + H331-H312-H315-H317-H319-H334-H351-H361d-H411|
|Precautionary statements||P261-P273-P280-P301 + P310-P305 + P351 + P338-P311|
|RIDADR||UN 3316 9|
Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory
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|D8537||Dulbecco’s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture|
|C2978||CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.|
|A3221||Iodoacetamide, vial of 56 mg|
Tributylphosphine solution, 200 mM (in N-
|MITOISO1||Mitochondria Isolation Kit, sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues|
|CYTOCOX1||Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria|
|CS0760||Isolated Mitochondria Staining Kit, kit sufficient for 50 reactions (in a 2 mL cuvette), kit sufficient for 1,000 reactions (using 96 multiwell plates)|
|P1644||Percoll®, pH 8.5-9.5 (25 °C)|
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