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MP0035 Sigma

LookOut® Mycoplasma PCR Detection Kit

Optimized for use with JumpStart Taq DNA Polymerase, D9307.

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Properties

Related Categories Assays & Reagents for Measuring Cytotoxicity, Proliferation & Viability Kits, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Culture, Mycoplasma Detection, Reagents and Supplements More...
quality   Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum
compatibility   Optimized for use with JumpStart Taq DNA Polymerase, D9307.
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Compatibility

The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

Application

The LookOut® Mycoplasma PCR Detection Kit has been used for screening of mycoplasma infection.

General description

The LookOut® Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals. Kit has been successfully tested for the ability to amplify Mycoplasma fermentans genomic DNA in concentrations of 20,000 to 20 copies.
The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. Total assay time is greatly reduced compared to general protocols that require individual loading of reaction tubes.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LookOut is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Mycoplasma Detection and Elimination

Mycoplasma contamination is a major headache for the cell culturist. Mycoplasma are small, simple bacteria which lack a cell wall and represent one of the most prevalent and serious sources of cell l...
Edward Burnett and Liz Penn, European Collection of Cell Culture (ECACC®)
Don Finley, Market Segment Manager, Sigma® Life Science
Biofiles, Vol. 8, No. 18
Keywords: Amplification, Antibiotics, Cell culture, Eliminations, Metabolism, Polymerase chain reaction

Mycoplasma Detection/Elimination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest concerns are mycoplasma contamination. Although mycoplasma do not usually kill contaminated cel...
Don Finley
BioFiles v7 n1, 2011, 14–15
Keywords: Eliminations, Metabolism, Polymerase chain reaction

Related Content

Optimized PCR-based Detection of Mycoplasma [VIDEO]

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest contaminant concerns are mycoplasma contamination. Although mycoplasma do not usually kill cont...
Keywords: Detection methods, Metabolism, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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Description

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D9307 JumpStart Taq DNA Polymerase, with MgCl2
08168 Timestrip Plus 8 °C

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