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  • N0379 - Nucleoside 5′-Diphosphate Kinase from baker's yeast (S. cerevisiae)

N0379 Sigma

Nucleoside 5′-Diphosphate Kinase from baker's yeast (S. cerevisiae)

lyophilized powder

Synonym: ATP:Nucleoside diphosphate phosphotransferase, NDPK



Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, 10-40% modified Warburg-Christian
storage temp.   −20°C
Gene Information   bakers yeast ... YNK1(853798)


General description

Nucleoside 5′-diphosphate kinase is a cytosolic enzyme.


1000, 2500 units in glass bottle


Reconstitute with deionized water. Solution is believed to be stable for over 1 year while frozen.

Unit Definition

One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.

Physical form

Lyophilized, essentially sulfate-free powder. Contains sodium citrate with a trace of magnesium and EDTA salts.


Nucleoside 5′-diphosphate kinase has been used in a study to examine a possible intracellular activity of the drug disodium cromoglycate in mast cells. It has also been used in a study to investigate protein synthesis in rabbit reticulocytes.

Biochem/physiol Actions

Nucleoside 5′-diphosphate kinase from Saccharomyces cerevisiae is found highly expressed in the cytoplasm. It affects DNA synthesis, in part, by binding to Cdc8p.

Transfers one phosphate from many nucleoside triphosphates to many nucleoside diphosphates. No activity when UMP is substituted for the diphosphate.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

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