• USA Home
  • N0379 - Nucleoside 5′-Diphosphate Kinase from baker's yeast (S. cerevisiae)

EMAIL THIS PAGE TO A FRIEND
N0379 Sigma

Nucleoside 5′-Diphosphate Kinase from baker's yeast (S. cerevisiae)

lyophilized powder

Synonym: ATP:Nucleoside diphosphate phosphotransferase, NDPK

Purchase

Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, 10-40% modified Warburg-Christian
storage temp.   −20°C
Gene Information   bakers yeast ... YNK1(853798)

Description

General description

Nucleoside 5′-diphosphate kinase is a cytosolic enzyme.

Packaging

1000, 2500 units in glass bottle

Reconstitution

Reconstitute with deionized water. Solution is believed to be stable for over 1 year while frozen.

Unit Definition

One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.

Physical form

Lyophilized, essentially sulfate-free powder. Contains sodium citrate with a trace of magnesium and EDTA salts.

Application

Nucleoside 5′-diphosphate kinase has been used in a study to examine a possible intracellular activity of the drug disodium cromoglycate in mast cells. It has also been used in a study to investigate protein synthesis in rabbit reticulocytes.

Biochem/physiol Actions

Nucleoside 5′-diphosphate kinase from Saccharomyces cerevisiae is found highly expressed in the cytoplasm. It affects DNA synthesis, in part, by binding to Cdc8p.

Transfers one phosphate from many nucleoside triphosphates to many nucleoside diphosphates. No activity when UMP is substituted for the diphosphate.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?