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N0409 Sigma

Anti-Nitrotyrosine antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   wide range
application(s)   immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain
  indirect ELISA: 1:1,000 using nitrated-BSA
  indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine
  microarray: suitable
  western blot: 1:1,000 using nitrated-BSA
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

General description

Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human diseases, such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury. In light of its highly reactive nature, evidence for a role of peroxynitrite in vivo is largely based on the detection of 3-nitrotyrosine, the major product of the peroxynitrite reaction, in injured tissues.

Immunogen

nitrated KLH (Keyhole Limpet Hemocyanin).

Preparation Note

Purified by affinity chromatography on a column of 3-nitro-L-tyrosine.

Specificity

Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, ρ-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates.

Reacts with peroxynitrite-nitrated proteins and 3-nitro-L-tyrosine. Does not crossreact with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine, and phospho-L-tyrosine. Binding of the antibody is blocked by 3-nitrotyrosine.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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