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N1127 Sigma

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

Synonym: o-Nitrophenyl β-D-galactopyranoside, ONPG

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Description

General description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Packaging

1, 5, 25 g in poly bottle

500 mg in poly bottle

250 mg in glass bottle

Principle

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Reconstitution

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

2-Nitrophenyl β-D-galactopyranoside

This product (ONPG) is a colorimetric substrate for β-galactosidase. This product is hydrolyzed to o-nitrophenol by the enzyme. o-Nitrophenol is yellow in alkaline solution and can be quantitated at ...
Keywords: Extinction coefficient, Melting

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Peer-Reviewed Papers
15

References

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