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N1377 Sigma

4-Nitrophenyl α-D-glucopyranoside

≥99%

Synonym: p-Nitrophenyl α-D-glucopyranoside

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 g in poly tube

5, 25 g in poly bottle

Price and Availability


Amplified Detection

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the solubility of 4-Nitrophenyl α-D-glucopyranoside, Product N1377?
We test the solubility of 4-Nitrophenyl α-D-glucopyranoside at 20 mg/mL in methanol.  When 4-Nitrophenyl α-D-glucopyranoside is used as a substrate for α-glucosidase, a 4 mM stock solution is made in water.
What is the melting point and specific rotation for 4-Nitrophenyl α-D-glucopyranoside, Product N1377?
The melting point range from our MSDS is 209-213 °C.  The optical rotation is lot specific, and can be found on the Certificate of Analysis at our website.
How is 4-Nitrophenyl α-D-glucopyranoside, Product N1377, used?
4-Nitrophenyl α-D-glucopyranoside is used as a substrate for the assay of α-glucosidase.  A stock solution of 4 mM is prepared in deionized water.  Upon the enzymatic hydrolysis of this substrate, 4-nitrophenol is produced.  The molar extinction coefficient of 4-nitrophenol in 0.01 M NaOH (pH 10.2) is approximately 18,380 at 400 nm. 
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Fermentation: Enzymes, Substrates and SCFA

The influence of probiotics on the metabolic activity of intestinal microbiota may be evaulated by detecting bacterial enzymatic activity in fecal samples, specifically β-glucosidase, β-glucuronidase...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Absorption, Cancer, Carcinogens, Cell proliferation, Chromatography, Enzyme activity, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Metabolites, Prebiotics, Probiotics, Thin layer chromatography

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Apoptosis, Cancer, Capillary electrophoresis, Carcinogens, Cell culture, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, Genomics, High performance liquid chromatography, Immunology, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Microbiology, Molecular biology, Nucleic acid hybridization, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Polymerase chain reaction - quantitative, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

Peer-Reviewed Papers
15

References

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