• USA Home
  • N1533 - Nitric Oxide Synthase, Endothelial bovine

EMAIL THIS PAGE TO A FRIEND
N1533 Sigma

Nitric Oxide Synthase, Endothelial bovine

recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution

Synonym: NOS III, ecNOS

Purchase

Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
recombinant   expressed in baculovirus infected Sf9 cells
form   buffered aqueous solution
activity   ≥0.3 units/mg protein
mol wt   mol wt 135 (kDa/subunit, homodimer)
shipped in   dry ice
storage temp.   −70°C
Gene Information   cow ... NOS3(287024)

Description

Unit Definition

One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 5 mM CHAPS, and 100 μM DTT.

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine and is constituitively expressed in endothelial cells (eNOS) and brain (nNOS).

Suitable for measuring NOS activity in the presence of exogenously added co-factors and/or inhibitors.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?