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N1907 Sigma

Depurination Solution for Neutral Southern Transfer

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Properties

grade   for molecular biology
form   liquid

Description

Application

Transfer of large DNA fragments (greater than 15kb) is problematic and inefficient. Often times, the agarose gel from which the DNA is being transferred dehydrates before complete transfer, thus trapping the DNA in the gel. Partial hydrolysis of the large DNA fragments can help to alleviate the problem of dehydration. An acid solution will produce a partial depurination of the DNA in an agarose gel. When followed by a strong base, the phosphodiester bond will hydrolyze at the depurination sites and allow the resulting DNA fragments (approx 1 kbp) to migrate through the gel to the membrane with greater ease allowing for a more efficient transfer.

Caution

It is important that depurination is not carried on to long otherwise the DNA fragments may become cleaved to short to bind well to the membrane and to hybridize strongly to the probe.

Other Notes

0.2 N HCl

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
UN 1760 8/PG 2

Documents

Certificate of Analysis

Protocols & Articles
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