Synonym: Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
|Related Categories||3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Antibody Characterization and Analysis, Antibody Glycan Analysis, Application Index,|
|Gene Information||Clostridium perfringens str. 13 ... nanJ(988807)|
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release. 3
Purified by affinity chromatography from Type VIII (N 5631)
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin unless otherwise specified.
Neuraminidase from Clostridium perfringens has been used in a study to assess purification via affinity chromatography. 1 It has also been used in a study to investigate site-directed mutations of amino acids of the neuraminidase gene, nanH. 2
Hydrolyzes α(2→3), α(2→6), and α(2→8)-glycosidic linkages of terminal sialic residues of various glycomolecules.
Neuraminidase can block attachment of type 3 reovirus to cell membranes. This effect is related to the ability of neuraminidase to hydrolysis sialic acid residues within cell surface receptors.
Customers Also Viewed
recombinant, expressed in E. coli, buffered aqueous solution, ≥250 units/mg protein
Proteomics Grade, suitable for MALDI-TOF MS
Type V, lyophilized powder
Type VI, lyophilized powder, 6-10 units/mg protein (using NAN-lactose), 2-5 units/mg protein (mucin)
Type VIII, lyophilized powder, 10-20 units/mg protein (using NAN-lactose), 3.5-8.0 units/mg protein (mucin)
Certificate of Analysis
Certificate of Origin
2.1. This procedure applies to products that have a specification for neuraminidase content by enzymatic determination.
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
1. Purification of neuraminidases from Vibrio cholerae, Clostridium perfringens and influenza virus by affinity chromatography Cuatrecases, P. and G. Illiano Biochem. Biophys. Res. Commun. 44, 178-84, (1971)
2. Site-directed mutations of the catalytic and conserved amino acids of the neuraminidase gene, nanH, of Clostridium perfringens ATCC 10543 Chin-Hsiang, C., et al. Enzyme Microb. Technol. 19, 267-76, (1996)
Effects of substitutions in the binding surface of an antibody on antigen affinity Dougan, D.A., et al Protein Eng. 111(1), 65-74, (1998)
A single amino acid alteration in the human parainfluenza virus type 3 hemagglutinin-neuraminidase glycoprotein confers resistance to the inhibitory effects of zanamivir on receptor binding and neuraminidase activity. Murrell, M.T., et al J. Virol. 75(14), 6310-6320, (2001)
Mutations in Human Parainfluenza Virus Type 3 Hemagglutinin-Neuraminidase Causing Increased Receptor Binding Activity and Resistance to the Transition State Sialic Acid Analog 4-GU-DANA (Zanamivir) Murrell, M., et al J. Virol. 77(1), 309-317, (2003)
Triggering of Human Parainfluenza Virus 3 Fusion Protein (F) by the Hemagglutinin-Neuraminidase (HN) Protein: an HN Mutation Diminishes the Rate of F Activation and Fusion Porotto, M., et al J. Virol. 77(6), 3647-3654, (2003)
Neuraminidase inhibitors for influenza: a review and public health perspective in the aftermath of the 2009 pandemic. Beck CR, Sokal R, Arunachalam N, et al. Influenza Other Respi. Viruses 7 Suppl 1, 14-24, (2013)
In situ molecular identification of the influenza A (H1N1) 2009 Neuraminidase in patients with severe and fatal infections during a pandemic in Mexico City. Ocadiz-Delgado R, Albino-Sanchez ME, Garcia-Villa E, et al. BMC Infect. Dis. 13, 20, (2013)
Pathway Pattern-based prediction of active drug components and gene targets from H1N1 influenza's treatment with maxingshigan-yinqiaosan formula. Dai W, Chen J, Lu P, et al. Mol. Biosyst. 9(3), 375-85, (2013)
NA proteins of influenza A viruses H1N1/2009, H5N1, and H9N2 show differential effects on infection initiation, virus release, and cell-cell fusion. Chen Q, Huang S, Chen J, et al. PLoS ONE 8(1), e54334, (2013)
What is the role of respiratory viruses in community-acquired pneumonia?: What is the best therapy for influenza and other viral causes of community-acquired pneumonia? Pavia AT Infect. Dis. Clin. North Am. 27(1), 157-75, (2013)
Identification of critical residues in the hemagglutinin and neuraminidase of influenza virus H1N1pdm for vaccine virus replication in embryonated chicken eggs. Wang W, Lu J, Cotter CR, et al. J. Virol. 87(8), 4642-9, (2013)
Polar residues and their positional context dictate the transmembrane domain interactions of influenza A neuraminidases. Nordholm J, da Silva DV, Damjanovic J, et al. J. Biol. Chem. 288(15), 10652-60, (2013)
Degradation, foraging, and depletion of mucus sialoglycans by the vagina-adapted Actinobacterium Gardnerella vaginalis. Lewis WG, Robinson LS, Gilbert NM, et al. J. Biol. Chem. 288(17), 12067-79, (2013)
Identification of selective inhibitors for human neuraminidase isoenzymes using C4,C7-modified 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA) analogues. Zhang Y, Albohy A, Zou Y, et al. J. Med. Chem. 56(7), 2948-58, (2013)
Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors RORγt and Ahr that leads to IL-17 production by activated B cells. Bermejo DA, Jackson SW, Gorosito-Serran M, et al. Nat. Immunol. 14(5), 514-22, (2013)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?