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N218 Sigma

Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse

clone NOS-3F7-B11-B5, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   rat, mouse, bovine
application(s)   immunohistochemistry (frozen sections): 1:100
  western blot: 1:50-1:500
clone   NOS-3F7-B11-B5, monoclonal
antibody form   ascites fluid
isotype   IgM
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Nos1(18125)
rat ... Nos1(24598)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

bovine cerebellum NOS.

Physical form

Ascites fluid containing ≤0.1% sodium azide

Specificity

The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).

Biochem/physiol Actions

Neuronal nitric acid synthase (Nos1) in involved in the production of nitric oxide. Mutations in the gene encoding it increases the susceptibility to behavioral dysfunction after an exposure to alcohol in mice.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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