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  • N2280 - Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse

N2280 Sigma

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse

clone NOS-B1, ascites fluid

Synonym: Anti-bNOS



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Neurotransmission,
species reactivity   human, goat, rat, pig
application(s)   microarray: suitable
  western blot: 1:3,000 using fresh rat cerebellum extract
clone   NOS-B1, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 150-160 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NOS1(4842)
rat ... Nos1(24598)
biological source   mouse
conjugate   unconjugated



recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

General description

Nitric oxide synthase is an enzyme that catalyzes the formation of nitric oxide. Nitric oxide is responsible for regulating several biochemical functions such as hemostasis, neurotransmission and vascular injury response . Mutations in nitric oxide synthase gene have been associated with diabetic nephropathy
Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is specific for nitric oxide synthase (NOS) derived from brain (bNOS, 150-160 kDa and several breakdown products of lower M.W.). The product does not bind to NOS derived from macrophages (mNOS) and endothelial cells (eNOS). This antibody is specific for bNOS in humans, goats, pigs and rats.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (3 papers)
Immunohistochemistry (2 papers)

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is suitable for use in microarray and western blot (1:3,000 using fresh rat cerebellum extract). The antibody may also be used in ELISA, immunoblot, immunohistochemistry (formalin-fixed, paraffin-embedded tongue tissue or paraformaldehyde perfusion-fixed brain tissue) and dot blot.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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