Synonym: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
|Related Categories||p-Nitrophenyl Phosphate Substrate Systems, Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric,|
Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Contains 20 mg substrate per tablet
Customers Also Viewed
powder, BioReagent, suitable for cell culture, ≥97%
for enzyme immunoassay, ≥99.0% (enzymatic)
Certificate of Analysis
Certificate of Origin
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
1. J.-P. Bretaudiere and T. Spillman, H.U. Bergmeyer, ed. Methods of Enzymatic Analysis 3rd ed., Weinheim, Fed.Rep.Ger. 4, 75, (1984)
2. O.H. Lowry Meth. Enzymol. 4, 366, (1957)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?