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N2783 Sigma

Nitric Oxide Synthase, Inducible from mouse

recombinant, expressed in E. coli, buffered aqueous solution

Synonym: Inducible Nitric Oxide Synthase, NOS II, iNOS, macNOS

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Properties

Related Categories 1.14.x.x Acting on paired donors with incorporation or reduction of molecular oxygen, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
recombinant   expressed in E. coli
form   buffered aqueous solution
activity   ≥4.0 units/mg protein
mol wt   mol wt 130 kDa (homodimer)
  mol wt 130 kDa (subunit, homodimer)
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... Nos2(18126)

Description

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine. iNOS is not calcium/calmodulin dependent and has a Km = 16 μM for L-arginine.

Unit Definition

One unit will produce 1.0 μmol of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 0.15 mM NADPH, 4.5 μM oxyhemoglobin, 18 μM tetrahydrobiopterin and 180 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 8 μM tetrahydrobiopterin

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

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Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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