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N3033 Sigma

Nitric Oxide Synthase, Neuronal from rat

≥95%, recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution

Synonym: NOS I, Neuronal Nitric Oxide Synthase, bNOS, ncNOS

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
recombinant   expressed in baculovirus infected Sf9 cells
assay   ≥95%
form   buffered aqueous solution
activity   ≥200 units/mg protein
mol wt   mol wt 150 (kDa/subunit, homodimer)
shipped in   dry ice
storage temp.   −70°C
Gene Information   rat ... Nos1(24598)

Description

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.

Unit Definition

One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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