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N3393 Sigma

Naphthol Blue Black

BioReagent, suitable for electrophoresis

Synonym: Acid Black 1, Amido Black 10B, Buffalo Black NBR

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Properties

Related Categories Molecular Biology, Other Stains for Electrophoresis, Protein Electrophoresis, Protein Stains and Staining Reagents, Proteomics More...
InChI Key   AOMZHDJXSYHPKS-DROYEMJCSA-L
product line   BioReagent
composition   Dye content, ~85%
impurities   ≤10% water
suitability   suitable for electrophoresis

Description

Packaging

25, 100 g in glass bottle

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QJ6196000
Protocols & Articles
Peer-Reviewed Papers
15

References

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