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Neural Stem Cells: Methods and Protocols

  •  ISBN-10 0-89603-964-1

  •  ISBN-13 978-0-89603-964-3

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Related Categories Books and Software, Cell Culture Books, Labware, Stem Cell Books
publication info   T. Zigova, et al., ed., Humana Press, 2002, 400 pp., hard cover
mfr. no.   Humana

Description

General description

Recognized experts describe the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. The techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. Protocols range from the simplest methods of isolation and characterization of neural cell properties to such sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.

Table of Contents

Part I. Isolation and Culture of NSCs
Neural Differentiation of Embryonic Stem Cells
Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens
Isolation of Stem and Precursor Cells from Fetal Tissue
Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb
Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium
Production of Immortalized Human Neural Crest Stem Cells
Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization
Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood
Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers
Part II. Characterization of NSCs In Vitro
A. Cellular Techniques
Analysis of Cell Generation in the Telencephalic Neuroepithelium
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
Assessing the Involvement of Telomerase in Stem Cell Biology
Detection of Telomerase Activity in Neural Cells
In Vitro Assays for Neural Stem Cell Differentiation
Electron Microscopy and Lac-Z Labeling
B. Electrophysiological Techniques
Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
C. Molecular Techniques
Fluorescence In Situ Hybridization
RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells
Differential Display: Isolation of Novel Genes
Cell Labeling and Gene Misexpression by Electroporation
Gene Therapy Using Neural Stem Cells
Modeling Brain Pathologies Using Neural Stem Cells
Part III. Utilization/Characterization of NSCs In Vivo
A. Endogenous Pools of Stem/Progenitor Cells
Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model
Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
Immunocytochemical Analysis of Neuronal Differentiation
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
B. Transplantation
Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS
Optimizing Stem Cell Grafting into the CNS
Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos
Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells
Routes of Stem Cell Administration in the Adult Rodent

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