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  • N3893 - Anti-Nitric Oxide Synthase, Endothelial (1185-1205) antibody produced in rabbit

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N3893 Sigma

Anti-Nitric Oxide Synthase, Endothelial (1185-1205) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-eNOS

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   bovine, mouse, rat, human
application(s)   immunohistochemistry (frozen sections): 1:100 using acetone-fixed, frozen tissue sections of mouse heart
  microarray: suitable
  western blot: 1:10,000 using bovine lung endothelial cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 135 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to nitric oxide synthase (NOS) of bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.

General description

The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated coronary artery disease.

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