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N5139 Sigma

Monoclonal Anti-Neurofilament 68 antibody produced in mouse

clone NR4, ascites fluid

Synonym: Monoclonal Anti-Neurofilament light chain

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurobiology,
species reactivity   pig, rat, chicken, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using rat cerebellum
  microarray: suitable
  western blot: 1:500 using bovine spinal cord neurofilament
clone   NR4, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen apparent mol wt 68 kDa
contains   15 mM sodium azide as preservative
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NEFL(4747)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

pig spinal cord.

General description

Intermediate filaments (IFs), with characteristic 10 nm diameter are a distinct class of heterogenous protein subunits apparent by both immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. The neurofilaments are one of the five major groups of IFs and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modification, which results in their heterogeneity, including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles.

Mouse Monoclonal clone NR4 anti-Neurofilament 68 antibody localizes the neurofilament of molecular weight 68,000 by indirect immunofluorescent labeling on formalin-fixed, paraffinembedded tissue sections. The antibody reacts specifically with neurofilament 68 in cultured cells or tissue preparations originating from human, pig, rat or chicken. It does not cross react with other intermediate filament proteins.

Application

Mouse Monoclonal clone NR4 anti-Neurofilament 68 antibody may be used for the localization of the neurofilament of molecular weight equal to 68,000 while being non-reactive with other intermediate filament proteins.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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