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N5386 Sigma

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym: Endonuclease micrococcal, MNase, Micrococcal endonuclease

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
composition   Protein, ≥40% E1%/280 (Balance primarily sodium acetate)
storage temp.   −20°C
Gene Information   Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Description

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Packaging

50, 200, 500 units in glass bottle

Application

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.

Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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