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N5389 Sigma

Monoclonal Anti-Neurofilament 200 antibody produced in mouse

clone NE14, ascites fluid

Synonym: Monoclonal Anti-Neurofilament heavy chain



Related Categories ALS, Prion Disease, and Others, ALS, Prion Disease, and others, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   guinea pig, chicken, mouse, bovine, pig, rat, human, rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
  immunohistochemistry (frozen sections): suitable
  microarray: suitable
  western blot: suitable
clone   NE14, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen apparent mol wt 200 kDa
contains   15 mM sodium azide as preservative
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NEFH(4744)
biological source   mouse
conjugate   unconjugated



pig spinal cord.

General description

Intermediate filaments (IFs), with characteristic 10 nm diameter are a distinct class of heterogenous protein subunits apparent by both immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. The neurofilaments are one of the five major groups of IFs and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modification, which results in their heterogeneity, including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles.

Mouse Monoclonal clone NE14 anti-Neurofilament 200 antibody specifically localizes the neurofilaments of molecular weight 200 kD usingindirect immunofluorescent labeling on formalin-fixed, paraffin-embedded tissue sections or immunoblottingprocedures. The antibody shows no reactivity with dephosphorylated neurofilaments. Good labeling may be obtained on human, pig, rat, chicken, bovine, guinea pig, rabbit and mouse tissue. The antibody does not cross react with the other intermediate filament proteins.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Mouse Monoclonal clone NE14 anti-Neurofilament 200 antibody may be used for the immunocytochemical localization of neurofilaments with molecular weights of 200 kD in cultered cells or tissue preparations.

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WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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