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N6019 Sigma

Neutralizing Buffer

for Northern and Southern blotting, powder

Purchase

Properties

Related Categories Buffer / Buffer Salts, Core Bioreagents, Life Science Reagents for Northern and Southern Blotting, Research Essentials
grade   for molecular biology
form   powder
foreign activity   DNase, RNase, none detected
storage temp.   room temp

Description

Components

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

General description

Sigma’s Neutralization Buffer for Southern procedures will neutralize the pH of the gel following depurination and denaturation treatment.

Application

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Articles

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Peer-Reviewed Papers
15

References

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GERPN3005 ECL Direct Nucleic Acid, GE Healthcare, RPN3005, pack of 1 ea
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

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