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N6261 Sigma

Nystatin

powder, BioReagent, suitable for cell culture

Synonym: Fungicidin, Mycostatin

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Properties

Related Categories Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antifungal,
Quality Level   PREMIUM
product line   BioReagent
form   powder
potency   ≥4,400 USP units per mg
solubility   H2O: insoluble
suitability   suitable for cell culture
antibiotic activity spectrum   molds
  yeast
storage temp.   −20°C

Description

Application

Nystatin is a polyene antibiotic. Its uses include preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in TLR2-expressing THP1 cells. This product is recommended for use in cell culture applications at 50 mg/L.

Biochem/physiol Actions

Mode of Action: Nystatin acts by binding to ergosterols in fungal cell membranes, increasing permeability of the membrane, and creating nystatin/ergosterol based ion channels. It also functions as a lipid raft-inhibiting reagent and as membrane associated cholesterol.

Antimicrobial spectrum: Neomycin acts against fungi, yeasts and molds.

Caution

Nystatin in tissue culuture media is stable at 37°C for three days. It is also stable in moderately alkaline media. Solutions and aqueous suspensions begin to lose activity soon after preparation, with heat, light, and oxygen accelerating the decomposition. Aqueous suspensions are stable for 10 minutes when heating to 100°C at pH 7.

Preparation Note

Nystatin is soluble at 28°C in methanol (11.2 mg/mL), ethanol (1.2 mg/mL), carbon tetrachloride (1.23 mg/mL), chloroform (0.48 mg/mL), benzene (0.28 mg/mL), and ethylene glycol (8.75 mg/mL). It is also soluble in DMSO at 5 mg/mL and freely soluble in DMF and formamide. It is not recommended to autoclave or sterile filter solutions of Nystatin. Nystatin is also effective as a suspension, stable at 37°C for 3 days. Prepare 50 mg/mL stock suspensions in water and store at -20°C.

Protocols & Applications

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Safety & Documentation

Safety Information

WGK Germany 
2
RTECS 
RF5950000

Protocols & Articles

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Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers

References

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Polyene toxicity in renal medulla: injury mediated by transport activity. Brezis, M., et al. Science 224, 66, (1984)

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Effect of selective decontamination on antimicrobial resistance in intensive care units: a systematic review and meta-analysis. Daneman N, Sarwar S, Fowler RA, et al. Lancet Infect. Dis. 13(4), 328-41, (2013)

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Mutational effects depend on ploidy level: all else is not equal. Gerstein AC Biol. Lett. 9(1), 20120614, (2013)

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Low expression of Kv7/M channels facilitates intrinsic and network bursting in the developing rat hippocampus. Safiulina VF J. Physiol. 586(Pt 22), 5437-53, (2008)

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Nystatin perforated patch recording and its applications to analyses of intracellular mechanisms. Akaike N and Harata N Jpn. J. Physiol. 44(5), 433-73, (1994)

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Fungal prophylaxis in very low birth weight neonates: nystatin, fluconazole or nothing? Isaacs D Curr. Opin. Infect. Dis. 21(3), 246-50, (2008)

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A reevaluation of nystatin in prophylaxis and treatment of oropharyngeal candidiasis. Alvarez Alvarez ME, Sánchez-Sousa A, and Baquero F Rev. Esp. Quimioter. 11(4), 295-315, (1998)

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Nystatin-rhodamine B assay for estimating activity of killer toxin from Kluyveromyces lactis. Palková Z and Vondrejs V Methods Mol. Biol. 53, 325-9, (1996)

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[What is nystatin?]. Gemeinhardt H Z. Arztl. Fortbild. (Jena.) 60(8), 503-6, (1966)

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Aqueous pores created in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B. Finkelstein A and Holz R Membranes 2, 377-408, (1973)

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Membrane dipole modifiers modulate single-length nystatin channels via reducing elastic stress in the vicinity of the lipid mouth of a pore. Chulkov EG, Schagina LV, and Ostroumova OS Biochim. Biophys. Acta 1848(1 Pt A), 192-9, (2015)

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Enhancement of tumor uptake and therapeutic efficacy of EGFR-targeted antibody cetuximab and antibody-drug conjugates by cholesterol sequestration. Chen Y, Liu G, Guo L, et al. Int. J. Cancer 136(1), 182-94, (2015)

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[Statement of the Polish Gynecological Society Expert Group on the use of Macmiror Complex 500]. Ginekol. Pol. 83(12), 956-9, (2012)

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Candidemia in Neurosurgical Intensive Care Unit: preliminary analysis of the effect of topical Nystatin prophylaxis and adequate source control on patients with early tracheostomy intubation. Giuliano S, Ceccarelli G, Renzini V, et al. Ann. Ig. 25(1), 57-64, (2013)

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In vitro antifungal activity of two tissue conditioners combined with nystatin, miconazole and fluconazole against Candida albicans. Chopde N, Pharande A, Khade MN, et al. J. Contemp. Dent. Pract. 13(5), 695-8, (2012)

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[New aspects of the pleiotropic action of the genes coding Saccharomyces cerevisiae exopolyphosphatases]. Gromosova EN, Kachur TL, Boĭchuk SI, et al. Mikrobiol. Z. 74(5), 26-31, (2012)

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Nystatin prophylaxis and treatment in severely immunodepressed patients. Gøtzsche PC and Johansen HK Cochrane Database Syst. Rev. (4), CD002033, (2002)

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Nystatin prophylaxis and treatment in severely immunodepressed patients. Gøtzsche PC and Johansen HK Cochrane Database Syst. Rev. (2), CD002033, (2002)

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Nystatin LF (Aronex/Abbott). Arikan S and Rex JH Curr. Opin. Investig. Drugs 2(4), 488-95, (2001)

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Nystatin prophylaxis and treatment in severely immunodepressed patients. Gotzsche PC and Johansen HK Cochrane Database Syst. Rev. (2), CD002033, (2000)

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Cholesterol dependence of collagen and echovirus 1 trafficking along the novel α2β1 integrin internalization pathway. Siljamäki E, Rintanen N, Kirsi M, et al. PLoS ONE 8(2), e55465, (2013)

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Endogenous VEGF is required for visual function: evidence for a survival role on müller cells and photoreceptors. Saint-Geniez, M PLoS ONE 3(11), e3554, (2008)

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Nystatin prophylaxis in immunocompromised children. Taylor TL Ann. Pharmacother. 30(5), 534-5, (1996)

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Peptide hormone ghrelin enhances neuronal excitability by inhibition of Kv7/KCNQ channels. Shi L, Bian X, Qu Z, et al. Nat. Commun. 4, 1435, (2013)

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Investigation of folate-conjugated fluorescent silica nanoparticles for targeting delivery to folate receptor-positive tumors and their internalization mechanism. Yang H, Lou C, Xu M, et al. Int. J. Nanomedicine 6, 2023-32, (2011)

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Biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei. Fjaervik E and Zotchev SB Appl. Microbiol. Biotechnol. 67(4), 436-43, (2005)

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Chemoprophylaxis of neonatal fungal infections in very low birthweight infants: efficacy and safety of fluconazole and nystatin. Blyth CC, Barzi F, Hale K, et al. J. Paediatr. Child Health 48(9), 846-51, (2012)

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Epstein-Barr virus transcytosis through polarized oral epithelial cells. Tugizov SM, Herrera R, and Palefsky JM J. Virol. 87(14), 8179-94, (2013)

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The effect of topically applied antimycotic agents on inner ear vestibular and cochlear function. Perez R, Nazarian Y, Sohmer H, et al. Laryngoscope 123(4), 1033-9, (2013)

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In vitro actinomycete biofilm development and inhibition by the polyene antibiotic, nystatin, on IUD copper surfaces. Shanmughapriya S, Francis AL, Kavitha S, et al. Biofouling 28(9), 929-35, (2012)

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Characterization of cortical neuronal and glial alterations during culture of organotypic whole brain slices from neonatal and mature mice. Jerome A. Staal et al PLoS ONE 6, e22040, (2011)

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Clinical use of oral nystatin in the prevention of systemic candidosis in patients at particular risk. Schäfer-Korting M, Blechschmidt J, and Korting HC Mycoses 39(9-10), 329-39, (1996)

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Isolating antifungals from fungus-growing ant symbionts using a genome-guided chemistry approach. Seipke RF, Grüschow S, Goss RJ, et al. Meth. Enzymol. 517, 47-70, (2012)

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Comparison of denture microwave disinfection and conventional antifungal therapy in the treatment of denture stomatitis: a randomized clinical study. Silva MM, Mima EG, Colombo AL, et al. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. 114(4), 469-79, (2012)

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Anti-diarrheal mechanism of the traditional remedy Uzara via reduction of active chloride secretion. Schulzke JD, Andres S, Amasheh M, et al. PLoS ONE 6(3), e18107, (2011)

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Sensitization of Candida albicans biofilms to various antifungal drugs by cyclosporine A. Shinde RB, Chauhan NM, Raut JS, et al. Ann. Clin. Microbiol. Antimicrob. 11, 27, (2012)

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Diverse host-seeking behaviors of skin-penetrating nematodes. Castelletto ML, Gang SS, Okubo RP, et al. PLoS Pathog. 10(8), e1004305, (2014)

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Peel bond strength of resilient liner modified by the addition of antimicrobial agents to denture base acrylic resin. Alcântara CS, Macêdo AF, Gurgel BC, et al. J. Appl. Oral Sci. 20(6), 607-12, (2012)

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Lectin-like oxidized LDL receptor-1 is palmitoylated and internalizes ligands via caveolae/raft-dependent endocytosis. Kumano-Kuramochi M, Xie Q, Kajiwara S, et al. Biochem. Biophys. Res. Commun. 434(3), 594-9, (2013)

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Uptake of different crystal structures of TiO₂ nanoparticles by Caco-2 intestinal cells. Gitrowski C, Al-Jubory AR, and Handy RD Toxicol. Lett. 226(3), 264-76, (2014)

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Prophylactic oral/topical non-absorbed antifungal agents to prevent invasive fungal infection in very low birth weight infants. Austin N, Darlow BA, and McGuire W Cochrane Database Syst. Rev. 3, CD003478, (2013)

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Sterol binding by methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants. Valitova J, Sulkarnayeva A, Kotlova E, et al. FEBS J. 281(8), 2051-60, (2014)

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The role of sterols in the lipid vesicle response induced by the pore-forming agent nystatin. Kristanc L, Božič B, and Gomišček G Biochim. Biophys. Acta 1838(10), 2635-45, (2014)

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Chitosan and gelatin based prototype delivery systems for the treatment of oral mucositis: from material to performance in vitro. Perchyonok VT, Zhang S, and Oberholzer T Curr. Drug Deliv. 10(1), 144-50, (2013)

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[Acute generalized exanthematous pustulosis, induced by nystatin]. Rosenberger A, Tebbe B, Treudler R, et al. Hautarzt 49(6), 492-5, (1998)

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Photodegradation study of nystatin by UV-Vis spectrophotometry and chemometrics modeling. Hemmateenejad B, Nekoeinia M, Absalan G, et al. J. AOAC Int. 97(4), 1206-12, (2014)

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[Clinical factors associated with invasive pulmonary aspergillosis in patients with chronic pneumopathies and respiratory isolation of Aspergillus spp]. Castón JJ, Linares MJ, Rivero A, et al. Med. Clin. (Barc.) 139(15), 668-71, (2012)

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Topical steroids for chronic wounds displaying abnormal inflammation. Bosanquet DC, Rangaraj A, Richards AJ, et al. Ann. R. Coll. Surg. Engl. 95(4), 291-6, (2013)

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The interaction of polysaccharide from Auricularia polytricha with quantum dots and the protection of plasmid DNA from damage. Wang W, Zhang G, and Zou J Appl. Biochem. Biotechnol. 169(8), 2263-72, (2013)

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HPLC and chemometric methods for the simultaneous determination of miconazole nitrate and nystatin. Heneedak HM, Salama I, Mostafa S, et al. J. Chromatogr. Sci. 50(10), 855-61, (2012)

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Free radical scavenging capacity, anticandicidal effect of bioactive compounds from Sida cordifolia L., in combination with nystatin and clotrimazole and their effect on specific immune response in rats. Ouédraogo M, Konaté K, Lepengué AN, et al. Ann. Clin. Microbiol. Antimicrob. 11, 33, (2012)

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Compounded preparations with nystatin for oral and oromucosal administration. Sklenár Z, Scigel V, Horácková K, et al. Acta Pol. Pharm. 70(4), 759-62, (2013)

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Prospective trial finds nystatin mouthwash effective prophylaxis for pulmonary invasive fungal infections that originate in the throat of patients with hematologic malignancies. Hu R, Jiang X, and Wu Y Neoplasma 60(3), 315-21, (2013)

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Nystatin. Witten VH and Katz SI Med. Clin. North Am. 54(5), 1329-37, (1970)

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Erythematous penile lesion. Noss MR and Cha J J. Fam. Pract. 61(12), 753-5, (2012)

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Nystatin prophylaxis for fungal peritonitis: to be or not to be? Lye WC Perit. Dial. Int. 27(5), 511-3, (2007)

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Merck 14,6736

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