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N6514 Sigma

Neuraminidase from Vibrio cholerae

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

Synonym: Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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Description

Application

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.

Preparation Note

A further purification by affinity chromatography of our Type III.

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride.

General description

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QQ3450000
Protocols & Articles

Protocols

Enzymatic Assay of Neuraminidase

2.1. This procedure applies to products that have a specification for neuraminidase content by enzymatic determination.

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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M8639 2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid sodium salt hydrate, ≥95% (HPLC)
B4666 5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt, ≥90%
N1516 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid, ≥95%

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