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N6760 Sigma

Nutrient Mixture F-12 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym: Ham’s F-12

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Properties

Related Categories Cell Culture, Classic Media and Salts, Nutrient Mixtures (Ham’s), Powder Media
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes
HEPES: no
storage temp.   2-8°C

Description

Application

Developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells.

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.176 g/L sodium bicarbonate.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Peer-Reviewed Papers
15

References

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