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N7271 Sigma

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Synonym: Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

.2 unit in poly bottle

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Preparation Note

Expressed in glycosidase-free hosts.

Unit Definition

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Price and Availability


Amplified Detection

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QQ3450000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What are the reaction conditions of the enzyme α(2→3) Neuraminidase, Product No. N7271?
One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally. This information also in the product datasheet on the website: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/4/n7271dat.Par.0001.File.tmp/n7271dat.pdf
What is the long term storage and shipping condition of α(2—›3) Neuraminidase, Product No. N7271?
It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.
For α(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?
The optimum pH range for α(2→3) neuraminidase is 6.0. Please see the data sheet. Here is the link to that document: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/4/n7271dat.Par.0001.File.tmp/n7271dat.pdf
What is the molecular weight of α(2—›3) Neuraminidase, Product No. N7271?
The molecular weight of this α(2→3) Neuraminidase enzyme is approximately 75 kDa.
How can I measure the enzyme activity of α(2—›3) Neuraminidase, Product No. N7271?
The assay procedure for this enzyme (E.C. 3.2.1.18) is found in this link below: http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/assays-by-enzyme-name-iii.html#- N -
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References

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