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N7653 Sigma

p-Nitrophenyl Phosphate Liquid Substrate System

liquid

Synonym: 4-Nitrophenyl phosphate disodium salt solution, pNPP

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Caution

Stable at least one year when stored below 0 °C.

Packaging

100 mL in glass bottle

Physical form

Ready-to-use.

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How should Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used?
Information on usage is found in our product information sheet located at our website. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/2/n7653pis.Par.0001.File.tmp/n7653pis.pdf
What type of application can Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used in?
This product is recommended for ELISA (microwell) procedures and is not recommended for membrane applications. Information on usage is found in our product information sheet located at our website. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/n7653pis.Par.0001.File.tmp/n7653pis.pdf \
What is the concentration of Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System?
The concentration of this product is considered proprietary.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

High Throughput Screening of Peptide Substrates for Tyrosine Kinases

Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism, and apoptosis. Therefore, identification of...
Keywords: Apoptosis, Enzyme-linked immunosorbent assay, Events, Infrared spectroscopy, Metabolism, Methods, Peptide synthesis, Phosphorylations, Sequences

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism and apoptosis. Those regulations are mediate...
Danhui Wang, Fan Zhang, Tao Zhao, Fei Zhong, Keming Song
Keywords: Apoptosis, Enzyme-linked immunosorbent assay, Events, Metabolism, Methods, Peptide synthesis, Pharmaceutical, Phosphorylations, Sequences

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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