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N7653 Sigma

p-Nitrophenyl Phosphate Liquid Substrate System

liquid

Synonym: 4-Nitrophenyl phosphate disodium salt solution, pNPP

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Caution

Stable at least one year when stored below 0 °C.

Packaging

100 mL in glass bottle

Physical form

Ready-to-use.

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, High performance liquid chromatography, Ion Exchange, Microscopy, Precipitation, Purification, Scanning electron microscopy

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

High Throughput Screening of Peptide Substrates for Tyrosine Kinases

Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism, and apoptosis. Therefore, identification of...
Keywords: Apoptosis, Enzyme-linked immunosorbent assay, Events, Infrared spectroscopy, Metabolism, Methods, Peptide synthesis, Phosphorylations, Sequences

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism and apoptosis. Those regulations are mediate...
Danhui Wang, Fan Zhang, Tao Zhao, Fei Zhong, Keming Song
Keywords: Apoptosis, Enzyme-linked immunosorbent assay, Events, Metabolism, Methods, Peptide synthesis, Pharmaceutical, Phosphorylations, Sequences

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Peptide synthesis, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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