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  • N7654 - Nuclease Methods and Protocols

N7654 Sigma

Nuclease Methods and Protocols

  •  ISBN-10 0-89603-679-0

  •  ISBN-13 978-0-89603-679-6



Related Categories Biochemistry Books, Books and Software, Labware More...
publication info   C.H. Schein, ed., Humana Press, 2001, 548 pp., hard cover
mfr. no.   Humana


General description

Leading scientists offer their methodologies for studying protein nucleases to determine their activity, structure, interaction with other molecules, and physiological role. Protocols range from methods for characterizing nucleases, to correlating nuclease structure and function, to the use of nucleases in cloning. Recombinant techniques for expressing and isolating nucleases are covered, as well as methods specific for dealing with protein/nucleic acid complexes. Each chapter contains a review of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and an explanation how the assay was developed.

Table of Contents

Part I. Characterizing Nuclease Activity.
Processivity of DNA Repair Enzymes,
Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribonucleases,
Ire1p: A Kinase and Site-Specific Endoribonuclease, .
Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases,
Quantitating mRNAs with Relative and Competitive RT-PCR,
Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants,
Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family,
Part II. Inhibitors and Activators of Nucleases.
Ribonuclease Inhibitors, .
Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon-g In Vitro,
Assays for the Evaluation of HIV-1 Integrase Inhibitors,
The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides,
The 2-5A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI),
Part III. Relating Nuclease Structure and Function.
Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein Complex, .
Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar,
Assaying In Vitro Refolding of RNases by Mass Spectrometry,
Dissecting Nucleases into Their Structural and Functional Domains: Mapping the RNA-Binding Surface of RNase III by NMR,
Using Electrostatics to Define the Active Site of Serratia Endonuclease,
Homology Modeling and Simulations of Nuclease Structures,
Methods for Determining Activity and Specificity of DNA Binding and DNA Cleavage by Class II Restriction Endonucleases,
Engineered Properties and Assays for Human DNase I Mutants,
Part IV. Nucleases in the Clinic.
Assays for Human DNase I Activity in Biological Matrices,
Evaluation of Retroviral Ribonuclease H Activity,
Assays for Detection of RNase A Superfamily Ribonucleases,
Assay for Antitumor and Lectin Activity in RNase Homologs, Kazuo Nitta. Isolation and Enzymatic Activity of Angiogenin,
Preparation and Preclinical Characterization of RNase-Based Immunofusion Proteins, .
Part V. Assays Using Nucleases.
Restriction Endonucleases and Their Uses,
Isolation and Characterization of an Unknown Restriction Endonuclease,
Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way,
Techniques to Measure Nucleic Acid-Protein Binding and Specificity: Nuclear Extract Preparations, DNase I Footprinting, and Mobility Shift Assays,
Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping,
Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays,

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