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  • N7782 - Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit

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N7782 Sigma

Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-iNOS

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   mouse, rat
application(s)   microarray: suitable
  western blot: 1:10,000 using cells extract of murine RAW 264.7 macrophages activated with LPS and IFN-γ.
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 130 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Nos2(18126)
rat ... Nos2(24599)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.

General description

Nitric Oxide Synthases (NOS) are enzymes involved in the synthesis of nitric oxide (NO), a messenger molecule generated under physiological conditions by virtually all mammalian cells. NO is formed from oxidation of L-arginine by NOS, releasing NO and L-citrulline. NO mediates diverse functions including vasodilation, synaptic neurotransmission, and cytotoxicity. NO participates in signal transduction pathways by activating soluble guanylate cyclase. At least three distinct isoforms of NOS have been described which are the products of different mammalian genes. The NOS isoform characterized in macrophages is a 130 kDa protein, also known as macrophage NOS (macNOS; mNOS), NOS2, or inducible NOS (iNOS). Inducible NOS is Ca2+ independent and is expressed in cytokine-activated macrophages and microglial cells in response to bacterial lipopolysaccharide (LPS) stimulation. iNOS not only occurs in macrophages but in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Nitric Oxide Synthase, Inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). By immunoblotting of an activated mouse macrophage RAW 264.7 cells extract, the staining of the iNOS band is specifically inhibited by the immunizing peptide.

Application

Rabbit polyclonal anti-Nitric Oxide Synthase is used to tag inducible nitric oxide synthase (iNOS) molecule for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting. It is used as a probe to determine the presence and roles of inducible nitric oxide synthase (iNOS) in a variety of cell signaling pathways via activation of enzymes such as guanylate cyclase.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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