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N7885 Sigma

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, sterile-filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

Synonym: Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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Description

Application

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Packaging

1 unit in poly bottle

2 units in poly bottle

Preparation Note

Chromatographically purified.

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.

Biochem/physiol Actions

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QQ3450000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Neuraminidase

2.1. This procedure applies to products that have a specification for neuraminidase content by enzymatic determination.

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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M8639 2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid sodium salt hydrate, ≥95% (HPLC)
B4666 5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt, ≥90%
N1516 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid, ≥95%

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